Journal: Scientific Reports
Article Title: Canine dorsal root ganglia satellite glial cells represent an exceptional cell population with astrocytic and oligodendrocytic properties
doi: 10.1038/s41598-017-14246-7
Figure Lengend Snippet: GFAP expression of canine ( a , c , e ) and murine ( b , d , f ) satellite glial cells (SGCs) supplemented with fibroblast growth factor 2 (FGF), epidermal growth factor, (EGF), ciliary neurotrophic factor (CNTF), heregulin 1β (HRG), and forskolin (Fors). ( a , b ) Graphs show the percentage of glial fibrillary acidic protein (GFAP) + SGCs with and without supplementation. ( c – f ) Immunofluorescence double-labelling of SGCs for 2′,3′-cyclic-nucleotide 3′-phosphodiesterase (CNPase; red) and GFAP (green) with bisbenzimide as nuclear counterstain. ( c , d ) control medium. ( e , f ) FGF supplementation. * P < 0.05. *** P < 0.001. Bars, 60 μm. Shown are means with standard errors of the mean (SEM).
Article Snippet: The proliferation rate of SGCs (passage 2) supplemented with fibroblast growth factor 2 (FGF-2, 40 ng/ml; recombinant human, Peprotech, Hamburg, Germany), epidermal growth factor (EGF, 40 ng/ml; R&D Systems, Wiesbaden-Nordenstadt, Germany), ciliary neurotrophic factor (CNTF; 40 ng/ml; Peprotec), heregulin 1β (HRG-1β, 40 ng/ml; R&D Systems), and the cAMP-elevator forskolin (4 µM; F6886; Sigma-Aldrich) was evaluated.
Techniques: Expressing, Immunofluorescence, Control